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KMID : 0903520070500010012
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 1 p.12 ~ p.17
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Physiological Characteristics of Biosurfactant-Producing Bacillus subtilis TBM 3101
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Kim Sun-A
Lee Young-Geun
Choi Yong-Lark
Hwang Cher-Won
Jeong Young-Kee
Joo Woo-Hong
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Abstract
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A biosurfactant-producing strain, Bacillus subtilis TBM 3101 was isolated from the soil sample at Tae-Baek Mountain through an antifungal test and emulsification assessment. The strain was assessed, regarding to the microbial growth, by physical and chemical test, surface tension, emulsification activity and stability. The surface tension of the isolate sharply decreased to the minimum 29 mN/m at 48 h growth. Of note, its emulsification was stabilized to the highest degree when tributyrin was utilized as a substrate, indicating that in comparison to a variety of synthetic surfactants, the biosurfactant produced by the isolate was significantly similar to synthetic surfactant, tween 20. In addition, the biosurfactant showed high emulsification activity when soybean oil, crude oil and tetradecane were used as a substrate. Thus, these studies could contribute to the detection and development of biosurfactant beneficial to the environment and humans.
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KEYWORD
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Bacillus subtilis, biosurfactant, emulsifying activity, emulsifying stability
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